ECDYSONE AND ECDYSONE ANALOGUES: THEIR ASSAY ON THE FLESHFLY Sarcophaga peregrina

Role of ecdysone, pupariation factors, and cyclic AMP in formation and tanning of the puparium of the fleshfly Sarcophaga bullata.

Two pupariation factors, anterior retraction factor (ARF) and puparium tanning factor (PTF), are absent from the hemolymph of larvae at the time of tanning accelerated by ARF/PTF, cyclic AMP, or dopamine. ARF and PTF are not involved in derepression of dopa decarboxylase (aromatic L-amino-acid decarboxylase, aromatic L-amino-acid carboxy-lyase, EC 4.1.1.28) synthesis initiated by ecdysone. Tann...

Identification of storage-protein messenger RNA of the fleshfly Sarcophaga peregrina.

Storage-protein mRNA was found to be abundant in poly(A)-containing RNA extracted from the fat-body of third-instar larvae of Sarcophaga peregrina (fleshfly). This RNA sedimented at the position of 19S on sucrose-density-gradient centrifugation and the product of its translation in vitro was 75K protein (protein of mol.wt. 75 000), which was precipitated specifically with antibody against stora...

The Ecdysone Receptor Puzzle

The present article reviews some recent findings on the functional ecdysone † receptor which is a heterodimer of two proteins: ecdysone receptor (EcR) and Ultraspiracle (USP). Emphasis is given to some unique aspects of this receptor, in particular to its dimerization, binding to DNA, and transactivation capabilities. The effects of ligands (ecdysone, juvenile hormone) on these functions are di...

Responses to Ecdysone Express D ifferent Ecdysone Receptor lsoforms

In D. melanogaster a pulse of the steroid hormone ecdysone triggers the larval-to-adult metamorphosis, a complex process in which this hormone induces imaginal t issues to generate adult structures and larval tissues to degenerate. We show that the EcR gene encodes three ecdysone receptor isoforms (EcR-A, EcR-81 , and EcR-B2) that have common DNAand hormone-binding domains but different N-termi...

Functional Analysis of Ecdysone Receptor